Free Sample- LED Light for Microscope

 

Key Features

Exceeds the brightness of 100W Mercury Lamp
Cool light
Instant ON/OFF. No warm-up or cool-down required
Adjust light output
Easy installation
 

 

Wavelength Range: 350-780nm
External Power Supply: Universal input 110-240V, 50/60Hz
Related Fluorescence: DAPI, GFP/FITC, Texas Red, Cy3
Sample LED FWHM: 454/24.8nm  523/36nm  368/16nm
Power Consumption: 40W
Dimensions HEAD: 142×95×85mm (L×W×H)
Dimensions TOUCH PAD: 130×126×20mm (L×W×H)
LED ON/OFF Response Time: 1ms
I/O Connector: PS/2
Control method: RS-232
LED Life Time: 30000 hours

   

 

All mercury arc lamps used a common heat absorbing filter from Schott…the
KG1. Further, all Ploem illuminators (still today) used a RED absorbing filter,
again from Schott, the BG-38 blue glass. These two filters are normally
removed from the light path when one is working in the near UV range using
filter block 400nm.
I have measured the temperature of the illumination beam of the HBO 100W
bulb using an analog "meat thermometer" with probe tip. I have measured the
temperature of the collimated beam immediately after the KG1 heat filter and
recorded temperatures in the range from 85-95F depending on placement
position. I then measured the temperature down on the stage of the
microscope with both the BG-38 and filter cube in position (no objective lens).
Filters cubes used were the H (Blue light 510 block) and N(Green light 580
block). I found that the temperature range on the surface of the microscope
stage where the slide would be placed was only a few degrees above room
temperature. The KG1 and BG38 filters along with the combined exciter filter
and emission (barrier) filters and filter blocks (dichroic beam splitters) were
doing their job nicely.
One problem encountered by those doing epifluorescence microscopy using the
arc lamp is "quenching" of the fluorescent dye (fluorochrome). "Fading" of the
emitted light during exposure became somewhat of a problem. Certain "anti?fade" tricks applied during sample prep became a useful solution to some
extent. Certainly the advantage of using LED as an alternative to mercury arc
lamps is now realized. One turns on the LED (optimized for the four dichroic
blocks 400, 455, 510 and 580nm) and then simply reduces intensity as you
would with a halogen bulb. LED illumination is not as "cool" as you would
think. The same heat and red absorbing filters are called for. "Fading" (and
temperature) is then reduced by adjusting the intensity of the LED. No warm up
or shutdown problems with LED. No shutters are needed in the light path. The
LED can easily be controlled via connection to your computer. LED illumination
lasts 20-50,000 hours. Mercury arc bulbs last about 200 hours and cost about
$200.00 each. Do the math and accept the performance enhancements found
with modern LED illumination. We no longer use silver halide for
documentation in microscopy….we are in a digital world now and the LED
promises to be the most efficient light source for optical light microscopy
fluorescence studies. Contact FSM with any questions. We have the best
alternative LED lighting now for taking the place of mercury arc illumination.
 

 

With 6 years working, we succesfully get a good LED illuminator for microscope. It provides stable performance and over 20000 hours lifetime.
Max 9 channels in a device.