Salmonella RTPCR Machines Test Kits

Here is a professional technical specification summary for Salmonella RT-PCR Test Kits (96 tests/kit) from (SureTect / MicroSEQ) and typical

(Molecular Detection / ANSR) systems. You can directly use this in your catalog / quotation / datasheet

🧪 Salmonella RT-PCR Test Kit – 96 Tests (Specification)

🔬 1. (SureTect / MicroSEQ)

✅ Product Example:

• SureTect™ Salmonella Species PCR Assay (96 tests/kit)
• MicroSEQ® Salmonella spp. Detection Kit

📦 General Kit Configuration (96 Tests)

• Total Tests: 96 reactions per kit
• Format: Pre-filled PCR tubes / lyophilized reagents
• Includes:
  • PCR reaction tubes (Salmonella specific primers & probes)
  • Lysis reagents / Proteinase K
  • Internal Positive Control (IPC)
  • Caps / sealing films
• Compatible with: 96-well real-time PCR systems

⚙️ Technical Specifications

🧬 Detection Target

• Detects: Salmonella spp. (all major serotypes)
• Optional multiplex detection:
  • S. Typhimurium
  • S. Enteritidis
  • S. Infantis

👉 Uses DNA amplification with real-time PCR (qPCR) technology (PMC)

⏱️ Time to Result

• Total workflow: 10–24 hours (including enrichment) (SelectScience)
• PCR run time: ~ 40 minutes

📊 Sensitivity

• Detects as low as:
  • 1–3 CFU per 25 g sample (after enrichment)

🧪 Throughput

• Up to 96 samples per run (Fisher Scientific)

🖥️ Instrument Compatibility

• Applied Biosystems™:
  • QuantStudio™ 5 Food Safety PCR
  • 7500 Fast Real-Time PCR System
• Software:
  • RapidFinder™ / RapidFinder Express

🔒 Validation & Compliance

• AOAC Performance Tested Methods
• Compatible with:
  • ISO 6579 (Salmonella detection)
  • FDA BAM methods

🔁 Workflow Steps

1. Sample enrichment (BPW media)
2. DNA extraction (PrepSEQ kit)
3. Real-time PCR amplification
4. Automated result interpretation

🌍 Applications

• Food industry (meat, dairy, poultry, spices)
• Environmental swabs
• Feed & agriculture testing

🧪 2. (Molecular Detection / ANSR)

✅ Product Example:

• ANSR® / Molecular Detection Salmonella Kit (96 tests)

📦 Key Specifications

🧬 Technology

• Real-time DNA amplification (isothermal / PCR-based depending on model)

⏱️ Time to Result

• 8–24 hours total
• Amplification time:
  • 20–60 minutes (rapid systems)

📊 Sensitivity

• Comparable to PCR methods:
  • Detects low CFU levels after enrichment

⚙️ Features

• Ready-to-use reagent tubes
• Internal control included
• Minimal sample prep
• Portable / compact instruments (ANSR system)

🖥️ Instrument Compatibility

• ANSR® System
• Molecular Detection System (MDS)

📜 Validation

• AOAC / ISO validated methods
• Widely accepted in food safety labs

📦 Storage & Handling (Both Brands)

• Storage Temperature: 2–8°C (some components frozen)
• Shelf Life: ~ 9–12 months
• Cold chain required

📌 Packaging Details (Export Ready)

• Kit Size: 96 tests
• Packing: Insulated box with ice packs
• HS Code: 3822 (Diagnostic reagents)
• Shelf life label & COA included